Fluxor assay
WebThe FluxOR™ Potassium Ion Channel Assay is an optically based, homogenous assay for high throughput screening (HTS) measurements of potassium ion channel and transporter activities. The homogenous assay is based on the permeability of potassium channels to thallium I. WebFor high-throughput screening of potassium ion channel and transporter activities we use the FluxOR™ II Green Potassium Ion Channel Assay (Thermo Fisher Cat# F20016) and …
Fluxor assay
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WebThe FluxOR™ Potassium Ion Channel Assay is an optically based, homogenous assay for high throughput screening (HTS) measurements of potassium ion channel and … WebFluxOR™ technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel activity, the FluxOR™ Potassium Ion Channel Assay is based on the activation of a novel fluorescent dye.
WebFluxOR™ thallium assay has been widely recognized for hERG activity test. This assay is optically based and can be performed on 96-well or 384-well plates as a homogenous assay for high-throughput screening (HTS) … WebThe FluxOR™potassium ion channel assay takes advantage of the permeability of potassium channels for thallium and uses the thallium influx to measure channel activity. …
WebFluxOR™ hERG Assay. Background and Service Details: hERG (human Ether-a-go-go-Related Gene, KCNH2) is a gene encoding alpha-subunit of a potassium channel. Properly formed and functional potassium channels are essential for normal electrical activity in the heart. Interference with the potassium channel due to the drug inhibition can result ... WebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye …
WebJul 29, 2024 · After 30 min of ATP stimulation (A) intracellular levels of K + was measured by the FluxOR II green potassium ion channel assay. BMDMs were either left untreated (0 mM) or treated with increasing concentrations of extracellular KCL (10 mM, 20 mM and 40 mM) and then infected with different CDC1551 Mtb strains (Mtb, ΔpknF mutant and …
WebAug 8, 2024 · Following the manufacturer’s procedure, which requires supplementation of assay and stimulus buffers with a proprietary background suppressor, FluxOR Red exhibited a 5.37 ± 0.001-fold increase in fluorescence at the highest concentration of ML297 compared to Thallos (4.30 ± 0.008-fold increase) and 8c (3.09 ± 0.004-fold increase). iron oak financial servicesWebScreening and assay development in a high throughput screening department; - Executed fluorescence, beta-lactamase and radioactive High Throughput Screens; focus on Calcium flux and membrane... iron oak crossfitiron oak energy servicesWebThe FluxOR assays take advantage of the well described permeability of potassium channels to thallium (Tl+) ions and the utilization of a new and improved indicator dye that results in a larger signal window and a significant improvement in … iron nutrition foundationWebA 5X dose-response series is added to the Na V 1.5 cells to block channel opening 15 minutes prior to addition of the EC 80 dose of veratridine. Results showed that FMP Red IC 50 was 6.2 μM and FMP Blue IC 50 was 6.3 μM. The change in fluorescence was equivalent between FMP Assays (Figure 2). port orford to brookings oregonWebMar 17, 2024 · The FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit … iron nutrition australia fact sheetWebMar 1, 2010 · FluxOR technology is a cell-based assay used for high-throughput screening measurements of potassium channel activity. Using thallium influx as a surrogate indicator of potassium ion channel... iron nutrition of plants in calcareous soils