Cisplatin cck8

Author: unwh

August undefined, 2024

Web Construction of cisplatin-resistant Siha cell line. (A) Morphology of Siha-N and Siha-DDP. (Magnifications, 200×). (B) Cell viability under the increasing concentration of cisplatin … WebCCK8 assays Cell viability was measured by cell counting kit-8 (CCK8). The cells were seeded into 96-well plates with 10 μL of CCK8 reagent in every well, and there were 4 × 104 cells in each well, all incubated at 37°C for 1 h. The optical density (OD) values were measured at 450-nm wavelength by Multiskan FC (Thermo Fisher Scientific, Inc.).

Elevated Cellular PD1/PD-L1 Expression Confers Acquired ... - PLOS

WebExosome-transmitted circVMP1 facilitates the progression and cisplatin resistance of non-small cell lung cancer by targeting miR-524-5p-METTL3/SOX2 axis Hongya Xie , Jie Yao , Yuxuan Wang & Bin Ni Pages 1257-1271 Received 24 Jan 2024, Accepted 14 Mar 2024, Published online: 25 Apr 2024 Download citation WebMay 28, 2024 · Non-Small Cell Lung Cancer (NSCLC) patients harboring EGFR activating mutations, such as in-frame deletions in exon 19 (Ex19del) or missense mutation in exon … high risk pregnancy nursing care plan

MiR-494 acts as a tumor promoter by targeting CASP2 in non

WebSep 30, 2024 · These cell strains were used to determine the effect of HOXB7 on cell viability, and cell apoptosis after exposure to cisplatin. CCK8 assay showed that treatment for 24 hours with cisplatin resulted in decreased IC50 of HOXB7‐knockdown cells compared with control cells (Fig (Fig3a). 3 a). This means that knockdown of HOXB7 … WebMar 15, 2024 · In vivo experiment showed that pretreatment with anemoside B4 effectively adjusted body weight and kidney index, and reduced cisplatin-elevated blood urea nitrogen (BUN) and creatinine (CREA) levels, as well as ameliorated the histopathological damage. WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … high risk pregnancy kansas city

National Center for Biotechnology Information

Cisplatin suppresses tumor proliferation by inhibiting autophagy in ...

WebFeb 8, 2024 · The rapid development of personalized medicine places high demands on the control of drug dose and cellular drug response to provide patients with better curative effects and low side effects. To solve the problem of low detection accuracies of the cell-counting kit-8 (CCK8) method, a detection method based on surface-enhanced Raman … WebNational Center for Biotechnology Information how many calories sweet potatoeWebFeb 16, 2024 · Generic name: cisplatin [ sis-PLA-tin ] Brand names: Platinol, Platinol-AQ, Platinol (Restricted Access) Dosage forms: intravenous powder for injection (50 mg), … how many calories the body burns a day

"WebJul 4, 2024 · To investigate whether miR‐320a regulates laryngeal carcinoma cell cisplatin resistance, miR‐320a overexpression HEp‐2 cells were treated with various concentration of cisplatin. CCK8 assays demonstrated that miR‐320a overexpression significantly inhibited HEp‐2 cell viability (Figure 4 E). " - Cisplatin cck8

Cisplatin cck8

HOXB7 mediates cisplatin resistance in esophageal squamous cell ...

WebCisplatin (CP) is a broad-spectrum antineoplastic agent, used to treat many different types of malignancies due to its high efficacy and low cost. However, its use is largely limited by acute... WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. Transfection of wild-type p53 gene enhanced the ...

Did you know?

WebObjective: To investigate the regulatory effect of miR-29b on gastric cells' resistance to cisplatin. Methods: The expression of miR-29b in gastric cancer cell line treated with cisplatin concentration gradient was detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and Western blotting. CCK8 was used to … WebCisplatin-induced acute kidney injury in mice was established by intraperitoneal injection of cisplatin. The levels of urea nitrogen and creatinine in serum were measured， renal pathology change was assayed by HE staining， qRT-PCR and Western blot were used to detect the expression levels of PIM1 mRNA and PIM1 protein.

WebNov 5, 2024 · Transcription factor knockdown and control cells were treated with cisplatin. CCK8 was used to detect cell viability and proliferation. Western blotting was … WebCCK8 was used to detect cell viability and proliferation. Western blotting was implemented to detect the levels of DNA repair-related proteins. Transwell assay was used to detect cell invasion. Results DNA repair was positively associated with the …

WebJan 12, 2024 · Parental and cisplatin-resistant HeLa cells were cultured in the presence of 1.0 μg/mL cisplatin in 96-well plates and stained with cell counting kit-8 (CCK8) at indicated time. Values are presented relative to cell viability on day 0. * p < 0.05, ** p < 0.01 compared with the parental cells; ( F ) Western blotting analysis of NHERF1 ... WebCCK8 assay was performed to assess cell proliferation and chemosensitivity of cisplatin-resistant A549/DDP and H1299/DDP cells. Colony-forming assay was utilized to detect colony numbers. Cell migration and invasion ability were measured by transwell assay. The protein levels of LRP, Pgp, MRP1, and SIX1 were examined by Western blot assay.

http://www.rx-8.com/chanpinzhongxin/203370.html

WebOct 22, 2024 · Cell viability was analyzed by the Cell Counting Kit-8 (CCK8). The relationship of Nrf2 and CD99 was assessed by dual-luciferase reporter gene assay and chromatin immunoprecipitation (ChIP). Bioinformatics analysis was performed to search for the downstream gene of CD99. high risk pregnancy kcWebCisplatin is a chemotherapy drug that contains the metal platinum. It stops or slows growth of cancer cells and other cells that grow rapidly by damaging their DNA. FDA Approved … high risk pregnancy maternal ageWebSep 18, 2024 · National Center for Biotechnology Information high risk pregnancy nursing diagnosisWeb目的探讨姜黄挥发油(TVO)联合顺铂对人皮肤鳞状细胞癌(鳞癌)A431细胞增殖、凋亡的影响及机制.方法取对数生长期A431细胞,用5、10、20、40、80 mg/L TVO处理,对照组用含有1％二甲基亚砜(DMSO)的DMEM高糖培养基处理,24 h后,CCK8法检测细胞增殖情况.另取部分A431细胞,分为4组,分别用含1％ DMSO的DMEM高糖培养基(对照 ... high risk pregnancy obgyn san antonioWebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often … how many calories to be in deficitWebMethods: We use CCK8 assay to show synergistic cytotoxicity of COE and cisplatin. Then, PI single staining and FITC-Annexin V/PI double staining were used to observe apoptotic cells through flow cytometry. The proteins of caspase signaling pathway were examined by Western blotting. high risk pregnancy percentageWebCCK8 assays were also utilized to detect IC50 values for cisplatin and 5-Fu. Results: The expression of circNRIP1 was significantly increased in the serum of chemoresistant NPC patients. At a functional level, we determined that circNRIP1 is able to sequester miR-515-5p, thereby inhibiting its ability to post-transcriptionally suppress IL-25 ... how many calories to break a fast